1D3J

N-TERMINAL DOMAIN CORE METHIONINE MUTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 32 2 1
Unit cell lengths	61.110, 61.110, 97.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.970
R-factor	0.164
Rwork	0.164
RMSD bond angle	0.020
Data reduction software	AUTOSTRAT
Data scaling software	SDMS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.770
High resolution limit [Å]	1.970 *	1.690
Rmerge	0.079	0.261
Number of reflections	21660
<I/σ(I)>	10
Completeness [%]	89.0	57
Redundancy	2.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.9	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol