1D2Y

N-TERMINAL DOMAIN CORE METHIONINE MUTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 32 2 1
Unit cell lengths	61.090, 61.090, 97.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.060
R-factor	0.163
Rwork	0.163
RMSD bond length	0.016
RMSD bond angle	2.660
Data reduction software	AUTOSTRAT
Data scaling software	SDMS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.940
High resolution limit [Å]	2.060 *	1.840
Rmerge	0.078	0.242
Number of reflections	16556
<I/σ(I)>	8.8
Completeness [%]	89.0	55
Redundancy	3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.9	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol