1D2U
1.15 A CRYSTAL STRUCTURE OF NITROPHORIN 4 FROM RHODNIUS PROLIXUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 105 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-12-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.920, 42.690, 52.750 |
| Unit cell angles | 90.00, 93.82, 90.00 |
Refinement procedure
| Resolution | 52.000 - 1.150 |
| R-factor | 0.13 |
| Rwork | 0.130 |
| R-free | 0.19000 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.040 | 0.260 |
| Total number of observations | 118648 * | |
| Number of reflections | 49629 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 90.0 | 74 |
| Redundancy | 2.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | Weichsel, A., (2000) Nat. Struct. Biol., 7, 551. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) |
