1D2I
CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE BGLII COMPLEXED WITH DNA 16-MER
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 1999-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9494, 0.9791, 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.800, 101.700, 116.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20000 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.880 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 * | 0.162 |
| Total number of observations | 629080 * | |
| Number of reflections | 123664 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.2 | 20 * | 15- 20% PEG 4000, 0.2 M AMMONIUM SULFATE, 0.1 M MES PH 5.2, MICROBATCH, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8-10 (mg/ml) | |
| 2 | 1 | 1 | PEG1540 | 17-20 (%) | |
| 3 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | 1 | MES | 0.1 (M) |
