1D2G
CRYSTAL STRUCTURE OF R175K MUTANT GLYCINE N-METHYLTRANSFERASE FROM RAT LIVER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-10 |
| Detector | MACSCIENCE DIP100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.700, 176.000, 45.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 28.200 * |
| Data reduction software | ELMS |
| Data scaling software | KUAVST |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.170 |
| Total number of observations | 71872 * | |
| Number of reflections | 22390 | |
| <I/σ(I)> | 3.35 | |
| Completeness [%] | 91.3 | 85 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | PEG-4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium citrate | 100 (mM) | |
| 2 | 1 | drop | ammonium acetate | 100 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | PEG4000 | 13 (%(w/v)) | |
| 5 | 1 | drop | protain | 10 (mg/ml) |
