1D2F
X-RAY STRUCTURE OF MALY FROM ESCHERICHIA COLI: A PYRIDOXAL-5'-PHOSPHATE-DEPENDENT ENZYME ACTING AS A MODULATOR IN MAL GENE EXPRESSION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-09-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.510, 107.190, 256.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.26200 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.420 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 | 0.303 |
| Total number of observations | 94388 * | |
| Number of reflections | 24839 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 94.9 | 88.7 |
| Redundancy | 3.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 * | 4 * | AMMONIUM SULPHATE, MES, TRIS, DITHITHREITOL, pH 7.0, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 3 (mM) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | drop | PLP | 0.020 (mM) | |
| 5 | 1 | reservoir | MES/Tris | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.8 (M) |
