1D28
CRYSTAL AND MOLECULAR STRUCTURE OF A DNA FRAGMENT: D(CGTGAATTCACG)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 183 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.780, 40.850, 65.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.17 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 0.038 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.087 * |
| Number of reflections | 2075 |
| Completeness [%] | 56.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA dodecamer | 1.22 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | magnesium chloride | 15 (mM) | |
| 4 | 1 | drop | spermine | 2.0 (mM) | |
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 22 (%) |
