1D21
BINDING OF THE ANTITUMOR DRUG NOGALAMYCIN AND ITS DERIVATIVES TO DNA: STRUCTURAL COMPARISON
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 61 |
| Unit cell lengths | 26.310, 26.310, 100.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 1.700 |
| R-factor | 0.208 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 0.054 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 3386 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 25 * | pH 5.00, VAPOR DIFFUSION, temperature 298.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 0.5 (mM) | |
| 2 | 1 | drop | 8 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 4 | 1 | drop | nogalamycin | 0.5 (mM) | |
| 5 | 1 | drop | PEG400 | 2 (%(w/v)) | |
| 6 | 1 | reservoir | PEG400 | 30 (%) |
