1D1Z

CRYSTAL STRUCTURE OF THE XLP PROTEIN SAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Detector	PRINCETON 2K
Spacegroup name	P 1
Unit cell lengths	32.480, 62.270, 65.090
Unit cell angles	65.71, 78.67, 89.66

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.139
Rwork	0.139
R-free	0.18700
RMSD bond length	0.011
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	22.000
High resolution limit [Å]	1.400
Rmerge	0.062
Total number of observations	274082 *
Number of reflections	83110
Completeness [%]	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	1.64 M AMMONIUM SULFATE, 100 MM SODIUM CITRATE, PH 5.6, AND 10 MM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	ammonium sulfate	1.64 (M)
6	1	reservoir	sodium citrate	100 (mM)
7	1	reservoir	dithiothreitol	10 (mM)