1D17
DNA-NOGALAMYCIN INTERACTIONS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 26.300, 26.300, 100.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 2.500 * - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| RMSD bond length | 0.018 * |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | Williams, L.D., (1990) Proc.Natl.Acad.Sci.USA, 87, 2225. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | METHANOL | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | SPERMINE | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.5 (mM) | single stranded |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.0 |
| 3 | 1 | drop | spermine | 4 (mM) | |
| 4 | 1 | drop | 13 (mM) | ||
| 5 | 1 | drop | MPD | 8 (%) | |
| 6 | 1 | drop | methanol | 5 (%sat) | with nogalamycin |
| 7 | 1 | reservoir | MPD | 30 (%) |
