1D14
STRUCTURE OF 11-DEOXYDAUNOMYCIN BOUND TO DNA CONTAINING A PHOSPHOROTHIOATE
Experimental procedure
| Temperature [K] | 283 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 17.730, 19.130, 26.360 |
| Unit cell angles | 90.00, 98.01, 90.00 |
Refinement procedure
| Resolution | 1.700 * - 1.500 |
| R-factor | 0.224 |
| RMSD bond length | 0.038 * |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 2129 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | pH 6.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.5 (mM) | single strand |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | 6.0 (mM) | ||
| 4 | 1 | drop | spermine | 1.5 (mM) | |
| 5 | 1 | drop | MPD | 20 (%) | |
| 6 | 1 | reservoir | MPD | 30 (%) |
