1D13
MOLECULAR STRUCTURE OF AN A-DNA DECAMER D(ACCGGCCGGT)
Experimental procedure
Temperature [K] | 283 |
Detector technology | DIFFRACTOMETER |
Detector | NICOLET P3 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 39.230, 39.230, 78.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.18 |
RMSD bond length | 0.026 |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 |
Number of reflections | 1434 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 * | VAPOR DIFFUSION |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA decamer | 1 (mM) | single strand |
2 | 1 | drop | sodium cacodylate | 40 (mM) | |
3 | 1 | drop | magnesium chloride | 13.0 (mM) | |
4 | 1 | drop | spermine tetrachloride | 5.0 (mM) | |
5 | 1 | drop | MPD | 5 (%) | |
6 | 1 | reservoir | MPD | 50 (%) |