1D11
INTERACTIONS BETWEEN AN ANTHRACYCLINE ANTIBIOTIC AND DNA MOLECULAR STRUCTURE OF DAUNOMYCIN COMPLEXED TO D(CPGPTPAPCPG) AT 1.2-ANGSTROMS RESOLUTION
Experimental procedure
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3X |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 27.860, 27.860, 52.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 * |
| R-factor | 0.175 |
| RMSD bond length | 0.034 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 9238 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA hexamer | (mM) | single strand | |
| 2 | 1 | daunomycin | 2 (mM) | ||
| 4 | 1 | spermine | 10 (mM) | ||
| 5 | 1 | cacodylate | 30 (mM) | ||
| 6 | 1 | 1 | MPD | 5 (%) |
