1D0X
DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH M-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1996-06-27 |
Detector | ADSC QUANTUM |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.000, 180.500, 54.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.187 |
Rwork | 0.187 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.830 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.039 | 0.105 |
Total number of observations | 190396 * | |
Number of reflections | 66106 | |
<I/σ(I)> | 31.4 | |
Completeness [%] | 95.2 | 96 |
Redundancy | 2.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7 | 277 | 8.3 % PEG 8000 125 MM NACL 3 MM DTT, pH 7.0, MICROBATCH, temperature 4.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 5 (mg/ml) | |
2 | 1 | 1 | PEG8000 | 8.3 (%) | |
3 | 1 | 1 | HEPES | 50 (mM) | |
4 | 1 | 1 | 125 (mM) | ||
5 | 1 | 1 | dithiothreitol | 3 (mM) |