1D0N

THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1994-09-14
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	169.400, 169.400, 154.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.2116
Rwork	0.205
R-free	0.23760
RMSD bond length	0.007
RMSD bond angle	1.339
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.077	0.246
Number of reflections	65683
<I/σ(I)>	23.7
Completeness [%]	92.0	82
Redundancy	3.44	2.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	291	ammonium sulfate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	34 (%sat)
2	1	reservoir	Tris-HCl	100 (mM)	pH8.0
3	1	drop	protein	10 (mg/ml)