1D0M

THE ESCHERICHIA COLI LYTIC TRANSGLYCOSYLASE SLT35 IN COMPLEX WITH BULGECIN A AND (GLCNAC)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-12-23
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	58.631, 67.751, 97.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.470
R-factor	0.179
Rwork	0.174
R-free	0.24100
RMSD bond length	0.007
RMSD bond angle	22.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.510
High resolution limit [Å]	2.470	2.470
Rmerge	0.037	0.118
Total number of observations	35069 *
Number of reflections	13028
<I/σ(I)>	24.8
Completeness [%]	90.3	69.3
Redundancy	2.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	295	BICINE-NAOH, ISOPROPANOL, PEG20K, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K