1D0B
INTERNALIN B LEUCINE RICH REPEAT DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-05-10 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.410, 56.920, 84.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.860 |
R-factor | 0.2103 |
Rwork | 0.207 |
R-free | 0.23500 * |
RMSD bond length | 0.004 |
RMSD bond angle | 1.160 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.910 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.046 | 0.196 |
Number of reflections | 18875 | |
<I/σ(I)> | 36.9 | 7.5 |
Completeness [%] | 98.5 | 89.7 |
Redundancy | 5.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES, 200 mM Calcium Acetate, 20% PEG 8000, 1 mM DTT , pH 6.5, vapor diffusion, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%) | |
2 | 1 | reservoir | 200 (mM) | ||
3 | 1 | reservoir | MES | 100 (mM) | |
4 | 1 | reservoir | dithiothreitol | 1 (mM) |