1D0B

INTERNALIN B LEUCINE RICH REPEAT DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-05-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.410, 56.920, 84.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.860
R-factor	0.2103
Rwork	0.207
R-free	0.23500 *
RMSD bond length	0.004
RMSD bond angle	1.160 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.910
High resolution limit [Å]	1.860	1.860
Rmerge	0.046	0.196
Number of reflections	18875
<I/σ(I)>	36.9	7.5
Completeness [%]	98.5	89.7
Redundancy	5.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	100 mM MES, 200 mM Calcium Acetate, 20% PEG 8000, 1 mM DTT , pH 6.5, vapor diffusion, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir		200 (mM)
3	1	reservoir	MES	100 (mM)
4	1	reservoir	dithiothreitol	1 (mM)