1D09
ASPARTATE TRANSCARBAMOYLASE COMPLEXED WITH N-PHOSPHONACETYL-L-ASPARTATE (PALA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-12-05 |
| Detector | UCSD MARK III |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 122.240, 122.240, 156.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.350 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.437 |
| Number of reflections | 74685 * | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 94.0 * | 90.7 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5.9 | 21 * | Krause, K.L., (1987) J.Mol.Biol., 193, 527. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 15-16 (mg/ml) | |
| 2 | 1 | 2 | maleic acid | 50 (mM) | |
| 3 | 1 | 2 | N-(phosphonacetyl)-L-aspartate | 1 (mM) | |
| 4 | 1 | 2 | 3 (mM) | ||
| 5 | 1 | 2 | N-ethylmorpholine |
