1D06

STRUCTURAL BASIS OF DIMERIZATION AND SENSORY MECHANISMS OF OXYGEN-SENSING DOMAIN OF RHIZOBIUM MELILOTI FIXL DETERMINED AT 1.4A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-20
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	60.600, 37.150, 53.990
Unit cell angles	90.00, 115.43, 90.00

Refinement procedure

Resolution	20.000 - 1.400
Rwork	0.224
R-free	0.27800 *
RMSD bond length	0.009
RMSD bond angle	1.479 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.150
High resolution limit [Å]	1.100	1.100
Rmerge	0.087	0.455
Total number of observations	216753 *
Number of reflections	41962
<I/σ(I)>	21.1
Completeness [%]	95.0	87.1
Redundancy	5.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	reservoir	sodium acetate	100 (mM)
3	1	reservoir	ammonium acetate	200 (mM)
4	1	reservoir	PEG4000	40 (%(w/v))