1CZV
CRYSTAL STRUCTURE OF THE C2 DOMAIN OF HUMAN COAGULATION FACTOR V: DIMERIC CRYSTAL FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.520, 70.540, 60.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1czs |
RMSD bond length | 0.006 |
RMSD bond angle | 1.390 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 2.510 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.094 | 0.399 |
Number of reflections | 14635 | |
Completeness [%] | 96.8 | 90.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 10 | 20 * | pH 10.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MOPS | 5 (mM) | pH8.0 |
2 | 1 | drop | protein | 6 (mg/ml) | |
3 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
4 | 1 | reservoir | ammonium sulfate | 0.7 (M) | pH9.5-10.0 |