1CZ9
ATOMIC RESOLUTION ASV INTEGRASE CORE DOMAIN (D64N) FROM CITRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.560, 66.560, 78.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| R-factor | 0.146 |
| Rwork | 0.147 |
| R-free | 0.15000 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | |
| Total number of observations | 345764 * | |
| Number of reflections | 53915 * | |
| <I/σ(I)> | 28.4 | |
| Completeness [%] | 97.1 | 90.4 |
| Redundancy | 6.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Bujacz, G., (1995) J. Mol. Biol., 253, 333. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | drop | protein | 6-7.5 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) |
