1CZ7
THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.194, 148.827, 261.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.29400 |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 0.280 * |
| Total number of observations | 136320 * | |
| Number of reflections | 48773 | |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 20 * | PEG 4000, NACL, MGCL2, K3PO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | 2 (M) | ||
| 11 | 1 | reservoir | dithiothreitol | 7 (mM) | |
| 2 | 1 | drop | PEG8000 | 6.8 (%) | |
| 3 | 1 | drop | 1 (M) | ||
| 4 | 1 | drop | ATP | 2 (mM) | |
| 5 | 1 | drop | dithiothreitol | 3.5 (mM) | |
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | drop | sodium phosphate | 25 (mM) | |
| 8 | 1 | reservoir | sodium phophate | 25 (mM) | |
| 9 | 1 | reservoir | PEG8000 | 13.5 (%) |
