1CZ1

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS AT 1.85 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	59.790, 64.540, 96.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.850
R-factor	0.163 *
Rwork	0.166
R-free	0.19600
RMSD bond length	0.011
RMSD bond angle	0.021
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.059 *	0.154
Number of reflections	32353 *
<I/σ(I)>	14.2
Completeness [%]	99.7	97.5
Redundancy	3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	292	Hepes buffer, CaCl2, Peg 8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	50 (mM)
2	1	drop		0.2 (M)
3	1	drop	PEG8000	20 (%(w/v))
4	1	drop	protein	10 (mg/ml)