1CYX
QUINOL OXIDASE (PERIPLASMIC FRAGMENT OF SUBUNIT II WITH ENGINEERED CU-A BINDING SITE)(CYOA)
Experimental procedure
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1992 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.000, 98.200, 39.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.300 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.31600 |
RMSD bond length | 0.010 |
RMSD bond angle | 26.270 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 1000.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.084 * |
Total number of observations | 17363 * |
Number of reflections | 7426 * |
Completeness [%] | 87.3 |
Redundancy | 3.19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | 4 * | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
4 | 1 | reservoir | acetic acid | 180 (mM) | |
5 | 1 | reservoir | sodium acetate | 20 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 50 (mM) | |
7 | 1 | reservoir | 1 (mM) |