1CY8

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	63.610, 79.270, 141.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 2.450
R-factor	0.225
Rwork	0.225
R-free	0.29500
RMSD bond length	0.006
RMSD bond angle	21.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.075	0.255
Total number of observations	418613 *
Number of reflections	27088
Completeness [%]	96.9	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	23 *	Lima, C.D., (1993) J.Mol.Biol., 232, 1213. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-10 (mg/ml)
2	1	drop		50 (mM)
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	dithiothreitol	1 (mM)