1CY7

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	63.830, 79.370, 141.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 2.400
R-factor	0.234
Rwork	0.234
R-free	0.29500
RMSD bond length	0.006
RMSD bond angle	21.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.208
Total number of observations	264320 *
Number of reflections	28970
Completeness [%]	89.0	87.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	4 *	and 14 degrees centigrade *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5-6 (mg/ml)
2	1	drop	nucleotide	1.45-20 (mM)
3	1	reservoir	ammonium sulfate	2.2-2.3 (M)