1CY6
COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 3' THYMIDINE MONOPHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.630, 80.720, 143.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.500 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.30900 |
RMSD bond length | 0.008 |
RMSD bond angle | 22.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 999.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.067 | 0.296 |
Total number of observations | 256045 * | |
Number of reflections | 26758 | |
Completeness [%] | 87.5 | 80.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | and 14 degrees centigrade * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-6 (mg/ml) | |
2 | 1 | drop | nucleotide | 1.45-20 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2.2-2.3 (M) |