1CY4

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'pTpTpTp3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	63.950, 78.760, 141.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 2.550
R-factor	0.221
Rwork	0.221
R-free	0.29900
RMSD bond length	0.006
RMSD bond angle	21.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.077	0.284
Total number of observations	335586 *
Number of reflections	24076
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	4 *	and 14 degrees centigrade *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5-6 (mg/ml)
2	1	drop	nucleotide	1.45-20 (mM)
3	1	reservoir	ammonium sulfate	2.2-2.3 (M)