1CXY
STRUCTURE AND CHARACTERIZATION OF ECTOTHIORHODOSPIRA VACUOLATA CYTOCHROME B558, A PROKARYOTIC HOMOLOGUE OF CYTOCHROME B5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.406, 46.406, 91.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.650 |
| R-factor | 0.186 |
| Rwork | 0.199 |
| R-free | 0.22000 |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.660 |
| High resolution limit [Å] | 1.650 | 1.600 |
| Rmerge | 0.059 * | 0.318 |
| Total number of observations | 92730 * | |
| Number of reflections | 14370 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 6.45 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | MES, NACL, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris/Cl | 10 (mM) | |
| 3 | 1 | reservoir | 4 (M) | ||
| 4 | 1 | reservoir | MES | 0.1 (M) |
