1CXU

1.42A RESOLUTION ASV INTEGRASE CORE DOMAIN FROM CITRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-31
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	66.177, 66.177, 78.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.420
R-factor	0.187
Rwork	0.187
R-free	0.22140
RMSD bond length	0.014 *
RMSD bond angle	0.029 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.046	0.050
Total number of observations	357298 *
Number of reflections	34287
<I/σ(I)>	15.7
Completeness [%]	99.0	98.9
Redundancy	5.55	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	277	Bujacz, G., (1995) J. Mol. Biol., 253, 333. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	isopropanol	10 (%)
3	1	drop	protein	6-7.5 (mg/ml)
4	1	reservoir	PEG4000	20 (%)