1CXU
1.42A RESOLUTION ASV INTEGRASE CORE DOMAIN FROM CITRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.177, 66.177, 78.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.420 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22140 |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 0.029 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.046 | 0.050 |
| Total number of observations | 357298 * | |
| Number of reflections | 34287 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 5.55 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | Bujacz, G., (1995) J. Mol. Biol., 253, 333. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | drop | protein | 6-7.5 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) |
