1CXE

COMPLEX OF CGTASE WITH MALTOTETRAOSE AT ROOM TEMPERATURE AND PH 9.1 BASED ON DIFFRACTION DATA OF A CRYSTAL SOAKED WITH ALPHA-CYCLODEXTRIN

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1992-01-20
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	121.388, 111.604, 66.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.153
R-free	0.19000 *
RMSD bond length	0.007
RMSD bond angle	17.500 *
Data reduction software	MADNES
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.200 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.051
Total number of observations	54298 *
Number of reflections	44448 *
Completeness [%]	82.9 *	36.1 *
Redundancy	1.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *		pH 9.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	28.7 (mg/ml)
2	1	drop	ammonium acetate	50 (mM)
3	1	drop	alpha-cyclodextrin	50 (mg/ml)
4	1	reservoir	MPD	60 (%(v/v))
5	1	reservoir	sodium Hepes	100 (mM)