1CXC

CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	82.330, 82.330, 37.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.225
RMSD bond length	0.010
RMSD bond angle	1.800
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	29.100
High resolution limit [Å]	1.530
Rmerge	0.073 *
Number of reflections	18286
Completeness [%]	91.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cytochrome c2	15 (mg/ml)
2	1	drop	MES	50 (mM)
3	1	drop	PEG4000	5 (%(w/v))
4	1	reservoir	MES	50 (mM)
5	1	reservoir	PEG4000	20 (%(w/v))
6	1	reservoir		0.6 (M)