1CXA
CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS
Experimental procedure
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.300, 64.300, 163.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.17 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 10854 |
| Completeness [%] | 74.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | imidazole | 100 (mM) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 70 (%sat) | |
| 4 | 1 | reservoir | imidazole | 100 (mM) |
