1CX8
CRYSTAL STRUCTURE OF THE ECTODOMAIN OF HUMAN TRANSFERRIN RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-09 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.400, 216.900, 361.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.24 * |
| Rwork | 0.243 |
| R-free | 0.28360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.099 | 0.337 |
| Number of reflections | 110528 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 82.0 | 32 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291 | 2.4 M KCl 10 mM KPi 1.5% PEG 20K, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 100 (mM) | ||
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | potassium phosphate | 5 (mM) | |
| 4 | 1 | drop | protein | 12.5 (mg/ml) | |
| 5 | 1 | reservoir | 2.3-2.5 (M) | ||
| 6 | 1 | reservoir | KPi | 10 (mM) | |
| 7 | 1 | reservoir | PEG6000 | 1.5-2.0 (%) | or 1.2-2.0& PEG20000 |
