1CX7
T4 LYSOZYME METHIONINE CORE MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.310, 61.310, 96.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.940 |
| R-factor | 0.152 |
| Rwork | 0.152 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.000 |
| Data reduction software | AUTOSTRAT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.900 * | 1.630 |
| Rmerge | 0.044 | 0.310 |
| Number of reflections | 23194 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 85.6 | 58.1 |
| Redundancy | 9.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 277 | Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | phosphate | 2.0 (M) | |
| 4 | 1 | reservoir | beta-mercaptoethanol |
