1CX6
T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.400, 61.400, 96.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.010 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.600 |
| Data reduction software | AUTOSTRAT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 16676 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 88.0 | 49 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 * | 277 | NA2PO4, NACL, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | 0.55 (M) | ||
| 3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | sodium phosphate | 0.01 (M) | |
| 6 | 1 | 1 | 2.2 (M) | ||
| 7 | 1 | 1 | 1.8 (M) |
