1CX4
CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 300.3 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-06 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 161.620, 161.620, 39.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.000 - 2.450 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.19800 |
RMSD bond length | 0.006 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.560 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.052 | 0.325 |
Number of reflections | 21965 | |
<I/σ(I)> | 14.3 | 3.94 |
Completeness [%] | 98.7 | 97.9 |
Redundancy | 4.182 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.75 | Diller, T.C., (2000) Protein Expression Purif., 20, 357. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 26 (mg/ml) | |
2 | 1 | reservoir | Tris | 80 (mM) | pH7.75 |
3 | 1 | reservoir | PEG3400 | 29 (%) | |
4 | 1 | reservoir | 80 (mM) |