1CWY
CRYSTAL STRUCTURE OF AMYLOMALTASE FROM THERMUS AQUATICUS, A GLYCOSYLTRANSFERASE CATALYSING THE PRODUCTION OF LARGE CYCLIC GLUCANS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-20 |
Detector | MARRESEARCH |
Spacegroup name | P 64 |
Unit cell lengths | 155.700, 155.700, 64.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 500.000 - 2.000 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22200 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 * | 0.337 * |
Total number of observations | 347606 * | |
Number of reflections | 56277 | |
<I/σ(I)> | 15.1 | 4.8 |
Completeness [%] | 94.0 | 93.8 * |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 291 | PEG 8000, ETHYLENE GLYCOL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 5 (mM) | |
2 | 1 | drop | dithiothreitol | 1 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | PEG8000 | 17 (%(w/v)) | |
6 | 1 | reservoir | HEPES | 100 (mM) | |
7 | 1 | reservoir | ethylene glycol | 10 (%(v/v)) |