1CWO
HUMAN CYCLOPHILIN A COMPLEXED WITH THR2, LEU5, D-HIV8, LEU10 CYCLOSPORIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-11-01 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.410, 57.440, 71.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.860 |
R-factor | 0.167 * |
R-free | 0.21500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cwa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.590 |
Data reduction software | XENGEN |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 |
Rmerge | 0.077 |
Total number of observations | 57971 * |
Number of reflections | 11317 |
Completeness [%] | 91.1 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 | used to cross-seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 50 (mM) | |
10 | 1 | reservoir | 0.02 (%) | ||
2 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
3 | 1 | drop | DMSO | 2 (%(v/v)) | |
4 | 1 | drop | 0.02 (%(w/v)) | ||
5 | 1 | drop | CypA | 0.5 (mM) | |
6 | 1 | drop | peptolide | 0.5 (mM) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
8 | 1 | reservoir | PEG8000 | 20 (%) | |
9 | 1 | reservoir | DMSO | 5 (%) |