1CWF
HUMAN CYCLOPHILIN A COMPLEXED WITH 2-VAL CYCLOSPORIN
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.400, 61.420, 72.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.860 |
R-factor | 0.177 |
Rwork | 0.177 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.590 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.860 * |
Rmerge | 0.053 * |
Total number of observations | 51113 * |
Number of reflections | 13548 * |
Completeness [%] | 95.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 295 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
10 | 1 | drop | CypA | 0.5 (mM) | |
2 | 1 | reservoir | PEG8000 | 19 (%(w/v)) | |
3 | 1 | reservoir | DMSO | 6 (%(v/v)) | |
4 | 1 | reservoir | 0.04 (%(w/v)) | ||
5 | 1 | drop | Tris-HCl | 50 (mM) | |
6 | 1 | drop | PEG8000 | 9.5 (%) | |
7 | 1 | drop | DMSO | 3 (%) | |
8 | 1 | drop | 0.02 (%) | ||
9 | 1 | drop | CsA-analogue | 0.5 (mM) |