1CWE

HUMAN P56LCK TYROSINE KINASE COMPLEXED WITH PHOSPHOPEPTIDE

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	35.170, 41.840, 45.520
Unit cell angles	68.71, 82.94, 87.36

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.192
Rwork	0.192
RMSD bond length	0.014
RMSD bond angle	1.800
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.300
Rmerge	0.060 *
Total number of observations	19230 *
Number of reflections	8054 *
Completeness [%]	93.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES/NaOH	10 (mM)
2	1	drop	PEG8000	13.5 (%(w/v))
3	1	drop		0.02 (%(w/v))
4	1	drop	Lck/peptide 2	2 (mM)
5	1	reservoir	HEPES/NaOH	20 (mM)
6	1	reservoir	PEG8000	27.0 (%(w/v))
7	1	reservoir		0.04 (%(w/v))