1CW7
LOW TEMPERATURE STRUCTURE OF WILD-TYPE IDH COMPLEXED WITH MG-ISOCITRATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.800, 102.800, 150.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.600 |
R-factor | 0.152 |
Rwork | 0.152 |
R-free | 0.22600 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 6.870 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.050 |
Total number of observations | 25364 * |
Number of reflections | 19141 |
<I/σ(I)> | 9.9 |
Completeness [%] | 75.1 |
Redundancy | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | MG-ISOCITRATE, AMMONIUM SULFATE, DTT, NAN3, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 38-55 (%sat) | |
2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
3 | 1 | reservoir | 0.02 (%(v/v)) | ||
4 | 1 | reservoir | meta-ligand solution | 1 (mM) |