1CW1
CRYSTAL STRUCTURE OF ISOCITRATE DEHYDROGENASE MUTANT K230M BOUND TO ISOCITRATE AND MN2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.800, 102.800, 150.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.530 - 2.100 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23500 * |
RMSD bond length | 0.006 |
RMSD bond angle | 23.700 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 5.530 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.056 | |
Total number of observations | 198526 * | |
Number of reflections | 40295 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 91.3 | 89.5 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | AMMONIUM SULFATE, DTT, NAN3, MN-ISOCITRATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 38-55 (%sat) | |
2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
3 | 1 | reservoir | 0.02 (%(v/v)) | ||
4 | 1 | reservoir | meta-ligand solution | 1 (mM) |