1CV6

T4 LYSOZYME MUTANT V149M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Spacegroup name	P 32 2 1
Unit cell lengths	60.870, 60.870, 97.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.159
Rwork	0.159
RMSD bond length	0.019
RMSD bond angle	2.600
Data reduction software	AUTOSTRAT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	1.950	1.950
High resolution limit [Å]	1.900 *	1.810
Rmerge	0.061	0.199
Number of reflections	17516
<I/σ(I)>	11.2
Completeness [%]	92.0 *	62.3
Redundancy	2.7	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol