1CUZ

CUTINASE, L81G, L182G MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	35.120, 67.360, 37.050
Unit cell angles	90.00, 93.90, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.129 *
Rwork	0.137
RMSD bond length	0.009 *
RMSD bond angle	1.430 *
Data reduction software	MARXDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.032
Total number of observations	33947 *
Number of reflections	10209
Completeness [%]	87.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	2-20 (%(w/v))
2	1	drop	HEPES	100 (mM)
3	1	drop	cutinase	5-15 (mg/ml)