1CUW

CUTINASE, G82A, A85F, V184I, A185L, L189F MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	130.400, 54.700, 69.840
Unit cell angles	90.00, 116.10, 90.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.141 *
Rwork	0.142
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MARXDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.158
Total number of observations	23137 *
Number of reflections	9151
Completeness [%]	94.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	2-20 (%(w/v))
2	1	drop	HEPES	100 (mM)
3	1	drop	cutinase	5-15 (mg/ml)
4	1	drop	MPD	15 (%)
5	1	reservoir	MPD	15 (%)