1CUQ
T4 LYSOZYME MUTANT V103M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.020, 61.020, 97.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.050 |
R-factor | 0.154 |
Rwork | 0.154 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.300 |
Data reduction software | AUTOSTRAT |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.990 |
High resolution limit [Å] | 1.800 | 1.840 |
Rmerge | 0.059 | 0.238 |
Number of reflections | 16482 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 88.9 | 54.3 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.7 * | 277 | Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | 0.25 (M) | ||
3 | 1 | reservoir | phosphate | 2.0 (M) | |
4 | 1 | reservoir | beta-mercaptoethanol |