1CUO
CRYSTAL STRUCTURE ANALYSIS OF ISOMER-2 AZURIN FROM METHYLOMONAS J
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-20 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.960, 33.670, 47.340 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.600 |
| R-factor | 0.184 |
| Rwork | 0.175 |
| R-free | 0.24200 |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.192 |
| Number of reflections | 12059 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 88.2 | 0.739 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Inoue, T., (1999) Acta Crystallogr., Sect.D, 55, 307. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 100 (mM) | |
| 3 | 1 | drop | PEG4000 | 12.5 (%(w/v)) | |
| 4 | 1 | reservoir | cacodylate | 100 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 25 (%(w/v)) |
