1CUD

CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM WITH THREE MOLECULES PER ASYMMETRIC UNIT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	65.900, 73.300, 71.200
Unit cell angles	90.00, 112.70, 90.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.151 *
Rwork	0.141
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	MARXDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.173
Total number of observations	79908 *
Number of reflections	16170
Completeness [%]	93.0
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	2-20 (%(w/v))
2	1	drop	HEPES	100 (mM)
3	1	drop	cutinase	5-15 (mg/ml)