1CU2

T4 LYSOZYME MUTANT L84M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Spacegroup name	P 32 2 1
Unit cell lengths	61.070, 61.070, 96.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.155
Rwork	0.155
RMSD bond length	0.018
RMSD bond angle	2.700
Data reduction software	AUTOSTRAT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.680	1.680
Rmerge	0.041
Number of reflections	21653
<I/σ(I)>	11.3
Completeness [%]	89.0	62.7
Redundancy	2.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol